7G9E
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102197-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.965, 70.965, 195.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.890 - 2.151 |
| R-factor | 0.2233 |
| Rwork | 0.220 |
| R-free | 0.29180 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.720 | 66.720 | 2.270 |
| High resolution limit [Å] | 2.150 | 6.800 | 2.150 |
| Rmerge | 3.511 | 0.098 | |
| Rmeas | 3.701 | 0.103 | 125.574 |
| Rpim | 1.124 | 0.032 | 40.361 |
| Total number of observations | 336793 | 10851 | 45590 |
| Number of reflections | 28098 | ||
| <I/σ(I)> | 4.1 | 14 | 0.5 |
| Completeness [%] | 99.8 | 99.9 | 99.4 |
| Redundancy | 12 | 10.3 | 11.5 |
| CC(1/2) | 0.486 | 0.996 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
