7G9C
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102141-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.094, 71.094, 195.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.530 - 2.686 |
| R-factor | 0.1984 |
| Rwork | 0.195 |
| R-free | 0.27420 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.810 | 66.810 | 2.830 |
| High resolution limit [Å] | 2.690 | 8.490 | 2.690 |
| Rmerge | 1.002 | 0.097 | |
| Rmeas | 1.025 | 0.100 | 12.665 |
| Rpim | 0.209 | 0.023 | 2.553 |
| Total number of observations | 339439 | 9897 | 49947 |
| Number of reflections | 14750 | ||
| <I/σ(I)> | 6.3 | 15.8 | 1.4 |
| Completeness [%] | 99.9 | 99.9 | 99.8 |
| Redundancy | 23 | 17.2 | 24 |
| CC(1/2) | 0.990 | 0.996 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
