7G9B
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102116-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.802, 70.802, 195.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.553 |
| R-factor | 0.2104 |
| Rwork | 0.206 |
| R-free | 0.29840 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.580 | 66.580 | 2.690 |
| High resolution limit [Å] | 2.550 | 8.070 | 2.550 |
| Rmerge | 1.107 | 0.091 | |
| Rmeas | 1.140 | 0.095 | 18.287 |
| Rpim | 0.270 | 0.025 | 4.247 |
| Total number of observations | 287818 | 8505 | 42754 |
| Number of reflections | 17061 | ||
| <I/σ(I)> | 4.9 | 16.8 | 0.7 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 16.9 | 13 | 17.7 |
| CC(1/2) | 0.986 | 0.997 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
