7G8X
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1416571195
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.176, 71.176, 195.858 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.830 - 1.711 |
R-factor | 0.2002 |
Rwork | 0.199 |
R-free | 0.22740 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.009 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.680 | 32.680 | 1.740 |
High resolution limit [Å] | 1.710 | 9.060 | 1.710 |
Rmerge | 0.389 | 0.057 | |
Rmeas | 0.396 | 0.058 | 13.843 |
Rpim | 0.075 | 0.012 | 2.586 |
Total number of observations | 1476697 | 9150 | 79110 |
Number of reflections | 54501 | ||
<I/σ(I)> | 7 | 41.4 | 0.4 |
Completeness [%] | 98.8 | 98.2 | 97.7 |
Redundancy | 27.1 | 19.7 | 28.1 |
CC(1/2) | 0.998 | 0.999 | 0.325 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |