7G8F
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1079168976
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.402, 71.402, 196.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.520 - 1.420 |
| R-factor | 0.1933 |
| Rwork | 0.192 |
| R-free | 0.21490 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.120 | 67.120 | 1.440 |
| High resolution limit [Å] | 1.420 | 7.780 | 1.420 |
| Rmerge | 0.167 | 0.045 | 5.476 |
| Rmeas | 0.170 | 0.046 | 5.681 |
| Rpim | 0.034 | 0.010 | 1.501 |
| Total number of observations | 2297755 | 15235 | 65442 |
| Number of reflections | 96982 | ||
| <I/σ(I)> | 11.4 | 67.2 | 0.4 |
| Completeness [%] | 100.0 | 100 | 99.6 |
| Redundancy | 23.7 | 20.5 | 14 |
| CC(1/2) | 0.999 | 0.999 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
