7G8E
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z104479710
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.588, 71.588, 196.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.170 - 1.790 |
R-factor | 0.1993 |
Rwork | 0.198 |
R-free | 0.22310 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.009 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.280 | 35.280 | 1.830 |
High resolution limit [Å] | 1.790 | 8.950 | 1.790 |
Rmerge | 0.341 | 0.070 | 5.345 |
Rmeas | 0.347 | 0.072 | 5.446 |
Rpim | 0.067 | 0.015 | 1.033 |
Total number of observations | 1307908 | 9504 | 75795 |
Number of reflections | 48815 | ||
<I/σ(I)> | 6.9 | 39.4 | 0.4 |
Completeness [%] | 99.3 | 98.8 | 98.5 |
Redundancy | 26.8 | 19.4 | 27.2 |
CC(1/2) | 0.998 | 0.999 | 0.348 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |