7G8A
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z100642432
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.341, 71.341, 196.493 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.670 - 1.500 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.21070 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.011 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.670 | 35.670 | 1.530 |
High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
Rmerge | 0.295 | 0.058 | 8.527 |
Rmeas | 0.301 | 0.060 | 8.761 |
Rpim | 0.059 | 0.013 | 1.983 |
Total number of observations | 2061779 | 12688 | 74795 |
Number of reflections | 82157 | ||
<I/σ(I)> | 8.6 | 49.7 | 0.5 |
Completeness [%] | 99.9 | 99.2 | 99 |
Redundancy | 25.1 | 20.2 | 18.7 |
CC(1/2) | 0.999 | 0.999 | 0.403 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |