7G87
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1148165337
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.626, 71.626, 197.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.810 - 2.053 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.24140 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.9) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 67.320 | 67.320 | 2.160 |
High resolution limit [Å] | 2.050 | 6.490 | 2.050 |
Rmerge | 0.644 | 0.097 | 7.101 |
Rmeas | 0.656 | 0.099 | 7.232 |
Rpim | 0.126 | 0.021 | 1.362 |
Total number of observations | 871788 | 26841 | 130925 |
Number of reflections | 33118 | ||
<I/σ(I)> | 6.3 | 22.2 | 0.7 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 26.3 | 21.9 | 27.7 |
CC(1/2) | 0.993 | 0.999 | 0.405 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |