7G84
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1102357527
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.342, 71.342, 196.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.420 - 1.811 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.23220 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.680 | 35.680 | 1.850 |
| High resolution limit [Å] | 1.810 | 9.060 | 1.810 |
| Rmerge | 0.339 | 0.067 | 3.259 |
| Rmeas | 0.354 | 0.071 | 3.404 |
| Rpim | 0.101 | 0.023 | 0.975 |
| Total number of observations | 564576 | 4056 | 32581 |
| Number of reflections | 47328 | ||
| <I/σ(I)> | 6.7 | 33.9 | 0.7 |
| Completeness [%] | 100.0 | 98.8 | 100 |
| Redundancy | 11.9 | 8.6 | 11.8 |
| CC(1/2) | 0.993 | 0.991 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
