7G1X
Crystal Structure of apo human FABP1 - monoclinic form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.691, 34.929, 59.321 |
Unit cell angles | 90.00, 119.21, 90.00 |
Refinement procedure
Resolution | 34.930 - 1.650 |
R-factor | 0.2051 |
Rwork | 0.202 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.017 |
RMSD bond angle | 2.038 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0018) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.930 | 34.930 | 1.690 |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.046 | 0.023 | 0.777 |
Rmeas | 0.055 | 0.028 | 0.993 |
Total number of observations | 77482 | ||
Number of reflections | 24662 | 315 | 1273 |
<I/σ(I)> | 13.85 | 35.07 | 1.17 |
Completeness [%] | 96.0 | 95.5 | 67.2 |
Redundancy | 3.142 | 3.01 | 1.932 |
CC(1/2) | 0.999 | 0.998 | 0.419 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368 |