7FZG
Crystal Structure of apo mouse FABP4, C-centered orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999980 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.801, 94.742, 49.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.370 - 1.490 |
R-factor | 0.1441 |
Rwork | 0.143 |
R-free | 0.17020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.019 |
RMSD bond angle | 2.008 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.370 | 47.370 | 1.530 |
High resolution limit [Å] | 1.490 | 6.660 | 1.490 |
Rmerge | 0.033 | 0.018 | 1.519 |
Rmeas | 0.039 | 0.020 | 1.646 |
Total number of observations | 198463 | ||
Number of reflections | 30207 | 398 | 2180 |
<I/σ(I)> | 21.92 | 73.59 | 1.43 |
Completeness [%] | 98.9 | 99 | 97.3 |
Redundancy | 6.54 | 5.872 | 6.692 |
CC(1/2) | 1.000 | 1.000 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368 |