7FYA
Crystal Structure of apo human FABP1 - orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 38.738, 56.431, 110.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.575 - 1.880 |
R-factor | 0.2181 |
Rwork | 0.215 |
R-free | 0.27390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.007 |
RMSD bond angle | 1.105 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_989) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.570 | 39.570 | 1.930 |
High resolution limit [Å] | 1.880 | 5.440 | 1.880 |
Rmerge | 0.049 | 0.026 | 0.787 |
Total number of observations | 119049 | ||
Number of reflections | 19160 | 961 | 952 |
<I/σ(I)> | 17.76 | 48.59 | 1.26 |
Completeness [%] | 93.2 | 99.2 | 77.3 |
Redundancy | 4.84 | 5.57 | 3.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368 |