7FWX
Crystal Structure of human FABP4 binding site mutated to that of FABP5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.700030 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 32.065, 52.722, 72.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.150 - 1.130 |
R-factor | 0.1748 |
Rwork | 0.172 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.018 |
RMSD bond angle | 2.006 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0018) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.170 | 36.150 | 1.160 |
High resolution limit [Å] | 1.130 | 5.050 | 1.130 |
Rmerge | 0.129 | 0.027 | 1.569 |
Rmeas | 0.138 | 0.029 | 1.705 |
Total number of observations | 298654 | ||
Number of reflections | 46726 | 624 | 3430 |
<I/σ(I)> | 9.11 | 45.9 | 1.32 |
Completeness [%] | 99.8 | 99.4 | 100 |
Redundancy | 6.43 | 5.736 | 6.593 |
CC(1/2) | 0.999 | 0.999 | 0.439 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368 |