7FPV
DHFR:NADP+:FOL complex at 290 K (crystal 5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 290 |
| Detector technology | PIXEL |
| Collection date | 2021-11-20 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.826533 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.196, 45.558, 99.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.730 - 1.040 |
| R-factor | 0.1269 |
| Rwork | 0.126 |
| R-free | 0.14500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7lvc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | DIALS (3.5.2) |
| Data scaling software | DIALS (3.5.2) |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.390 | 1.060 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.152 | 4.932 |
| Rpim | 0.031 | 1.101 |
| Number of reflections | 142372 | 7539 |
| <I/σ(I)> | 27.8 | 0.5 |
| Completeness [%] | 99.2 | |
| Redundancy | 26.4 | 20.7 |
| CC(1/2) | 0.999 | 0.364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 20 mM imadazole (pH 5.4-5.8), 16-21% PEG 400, 125 mM MnCl2 |
