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7FOY

PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E10 from the F2X-Universal Library

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsMAX IV BEAMLINE BioMAX
Synchrotron siteMAX IV
BeamlineBioMAX
Temperature [K]100
Detector technologyPIXEL
Collection date2020-06-12
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.999900
Spacegroup nameC 1 2 1
Unit cell lengths88.594, 82.157, 93.492
Unit cell angles90.00, 108.22, 90.00
Refinement procedure
Resolution41.080 - 1.550
R-factor0.2104
Rwork0.209
R-free0.25110
Structure solution methodFOURIER SYNTHESIS
Data reduction softwareXDS (Jan 10, 2022)
Data scaling softwareAimless
Phasing softwarePHENIX (1.17.1_3660)
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]41.08041.08041.080
High resolution limit [Å]1.5504.6201.550
Rmerge0.0442.689
Rmeas0.0710.0482.906
Total number of observations64276924723100736
Number of reflections92243356414628
<I/σ(I)>11.0942.840.27
Completeness [%]99.699.598.3
Redundancy6.976.946.89
CC(1/2)0.9990.9970.274
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.529319% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate

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