7FO1
PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G03 from the F2X-Universal Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.553, 82.076, 93.766 |
Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
Resolution | 41.040 - 1.500 |
R-factor | 0.2047 |
Rwork | 0.204 |
R-free | 0.22570 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.040 | 41.040 | 41.040 |
High resolution limit [Å] | 1.500 | 4.470 | 1.500 |
Rmerge | 0.043 | 2.235 | |
Rmeas | 0.061 | 0.047 | 2.410 |
Total number of observations | 705123 | 26736 | 109944 |
Number of reflections | 100703 | 3913 | 15727 |
<I/σ(I)> | 12.75 | 42.92 | 0.45 |
Completeness [%] | 98.3 | 98.8 | 95.1 |
Redundancy | 7 | 6.83 | 6.99 |
CC(1/2) | 0.999 | 0.997 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate |