7FNH
PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07C05 from the F2X-Universal Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.495, 81.959, 93.193 |
Unit cell angles | 90.00, 108.02, 90.00 |
Refinement procedure
Resolution | 43.590 - 1.570 |
R-factor | 0.2107 |
Rwork | 0.210 |
R-free | 0.24720 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.590 | 43.590 | 43.590 |
High resolution limit [Å] | 1.570 | 4.670 | 1.560 |
Rmerge | 0.054 | 2.520 | |
Rmeas | 0.076 | 0.059 | 2.730 |
Total number of observations | 609180 | 23403 | 92625 |
Number of reflections | 88654 | 3427 | 13769 |
<I/σ(I)> | 10.99 | 31.97 | 0.72 |
Completeness [%] | 99.2 | 99.5 | 95.9 |
Redundancy | 6.87 | 6.83 | 6.73 |
CC(1/2) | 0.997 | 0.994 | 0.429 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate |