7FMV
PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E12 from the F2X-Universal Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.617, 85.226, 92.690 |
Unit cell angles | 90.00, 110.53, 90.00 |
Refinement procedure
Resolution | 43.400 - 1.610 |
R-factor | 0.221 |
Rwork | 0.220 |
R-free | 0.25710 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.400 | 43.400 | 43.400 |
High resolution limit [Å] | 1.610 | 4.790 | 1.600 |
Rmerge | 0.037 | 2.524 | |
Rmeas | 0.077 | 0.040 | 2.739 |
Total number of observations | 567745 | 22190 | 85948 |
Number of reflections | 82874 | 3218 | 12904 |
<I/σ(I)> | 12.82 | 44.98 | 0.66 |
Completeness [%] | 99.3 | 99.5 | 96 |
Redundancy | 6.85 | 6.9 | 6.66 |
CC(1/2) | 0.999 | 0.999 | 0.306 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate |