7FJS

Crystal structure of T6 Fab bound to theSARS-CoV-2 RBD of B.1.351

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	97.103, 109.613, 162.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.730 - 2.900
R-factor	0.2204
Rwork	0.217
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.970
High resolution limit [Å]	2.900	7.150	2.900
Rmerge	0.227	0.073	0.918
Rmeas	0.251	0.082	1.011
Rpim	0.106	0.037	0.420
Total number of observations	215051
Number of reflections	39111	2761	2589
<I/σ(I)>	3.4
Completeness [%]	99.7	97.7	100
Redundancy	5.5	4.8	5.7
CC(1/2)		0.995	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	2.0 M ammonium sulfate, 5 % v/v 2-propanol