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7FJ8

KpAckA (PduW) with AMP complex structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL18U1
Synchrotron siteSSRF
BeamlineBL18U1
Temperature [K]100
Detector technologyPIXEL
Collection date2018-04-02
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.97915
Spacegroup nameC 2 2 21
Unit cell lengths102.098, 152.635, 131.743
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution43.040 - 2.100
R-factor0.178
Rwork0.177
R-free0.21460
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7fj7
RMSD bond length0.007
RMSD bond angle0.821
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.140
High resolution limit [Å]2.1002.100
Rpim0.0700.335
Number of reflections608172928
<I/σ(I)>27.683.41
Completeness [%]99.397.9
Redundancy12.19.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION72930.1 M Tris,10% (w/v) PEG-8000, 0.2M MgCl2, pH 7.0.

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