7FJ8
KpAckA (PduW) with AMP complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.098, 152.635, 131.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.040 - 2.100 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.21460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fj7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.821 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.070 | 0.335 |
Number of reflections | 60817 | 2928 |
<I/σ(I)> | 27.68 | 3.41 |
Completeness [%] | 99.3 | 97.9 |
Redundancy | 12.1 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Tris,10% (w/v) PEG-8000, 0.2M MgCl2, pH 7.0. |