7FJ7
KpAckA (PduW) native structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.865, 101.510, 90.794 |
Unit cell angles | 90.00, 125.19, 90.00 |
Refinement procedure
Resolution | 45.380 - 2.500 |
R-factor | 0.1835 |
Rwork | 0.181 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iir |
RMSD bond length | 0.008 |
RMSD bond angle | 0.939 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.032 | 0.211 |
Number of reflections | 38499 | 1895 |
<I/σ(I)> | 21.45 | 3.5 |
Completeness [%] | 98.8 | 97.2 |
Redundancy | 6.8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M BIS-TRIS, 45% v/v Polypropylene glycol P 400, pH 6.5. |