7FIW

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidAwMel(ST) and CidBND1-ND2 from wPip(Pel)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	82.357, 98.690, 184.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.620 - 2.160
R-factor	0.1926
Rwork	0.192
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fiv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.175	0.087	1.048
Rmeas	0.184	0.091	1.131
Rpim	0.054	0.027	0.406
Total number of observations	868431
Number of reflections	80310	4348	3524
<I/σ(I)>	5.1
Completeness [%]	98.8	99.7	88
Redundancy	10.8	11.7	6.2
CC(1/2)		0.997	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289.15	5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1M HEPES pH 7.5, 10% w/v Polyethylene glycol 10000