7FIU
Crystal structure of the DUB domain of Wolbachia cytoplasmic incompatibility factor CidB from wMel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.110, 60.590, 85.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.450 - 1.840 |
| R-factor | 0.2105 |
| Rwork | 0.209 |
| R-free | 0.23810 |
| Structure solution method | SAD |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.450 | 49.450 | 1.890 |
| High resolution limit [Å] | 1.840 | 8.230 | 1.840 |
| Rmerge | 0.081 | 0.056 | 1.063 |
| Rmeas | 0.086 | 0.061 | 1.126 |
| Rpim | 0.029 | 0.022 | 0.369 |
| Total number of observations | 190236 | 1973 | 14513 |
| Number of reflections | 22187 | 302 | 1590 |
| <I/σ(I)> | 14.3 | 26.5 | 2.1 |
| Completeness [%] | 99.4 | 98.9 | 98.6 |
| Redundancy | 8.6 | 6.5 | 9.1 |
| CC(1/2) | 0.998 | 0.996 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 10mM Nickel (II) Chloride hexahydrate, 100mM TRIS pH 8.5, 20% w/v Polyethylene Glycol Monomethyl Ether 2000 |
