7FIR
The crystal structure of beta-1,2-mannobiose phosphorylase in complex with 1,4-mannobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-11-01 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34138 |
| Spacegroup name | I 4 |
| Unit cell lengths | 205.303, 205.303, 78.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.290 - 2.200 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b0r |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.290 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.347 |
| Number of reflections | 161051 | 3738 |
| <I/σ(I)> | 8.9 | 3.1 |
| Completeness [%] | 99.2 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 10 mM ZnCl2, 28% PEG3350, 160 mM Tris-HCl pH 7.0 |
