7FIQ
The crystal structure of mannose-bound beta-1,2-mannobiose phosphorylase from Thermoanaerobacter sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-11-04 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34138 |
| Spacegroup name | I 4 |
| Unit cell lengths | 203.195, 203.195, 77.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.850 - 2.220 |
| R-factor | 0.1739 |
| Rwork | 0.173 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b0r |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 2.250 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.134 | 0.392 |
| Number of reflections | 150222 | 3065 |
| <I/σ(I)> | 9 | 3.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 10 mM ZnCl2, 24% PEG3350, 180 mM Tris-HCl pH 7.0 |
