7FI1

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in ManA bound form at pH-7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-04-07
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	48.139, 160.228, 46.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.723 - 2.430
Rwork	0.229
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.007
RMSD bond angle	1.504
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.760	2.530
High resolution limit [Å]	2.430	2.430
Rmerge	0.100	0.538
Number of reflections	13632	1217
<I/σ(I)>	20.24	1.99
Completeness [%]	94.8	76.7
Redundancy	12.8	2.47
CC(1/2)	0.993	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298.15	0.2M LiSo4 Monohydrate, 0.1M Sodium Cacodylate pH-7.0, 30%(w/v) PEG 8000