7FI0
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in ManA bound form at pH-5.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-05-13 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.54178 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 105.757, 160.662, 48.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.402 - 2.310 |
Rwork | 0.205 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fhu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.604 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.450 | 2.410 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.155 | 0.560 |
Number of reflections | 17710 | 1763 |
<I/σ(I)> | 10.04 | 2.14 |
Completeness [%] | 95.8 | 80.9 |
Redundancy | 6.39 | 2.88 |
CC(1/2) | 0.989 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298.15 | 0.2M LiSo4 Monohydrate, 0.1M Sodium acetate pH-5.0, 30% (w/v) PEG 3350 |