7FI0

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in ManA bound form at pH-5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-05-13
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54178
Spacegroup name	C 2 2 21
Unit cell lengths	105.757, 160.662, 48.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.402 - 2.310
Rwork	0.205
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.009
RMSD bond angle	1.604
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.450	2.410
High resolution limit [Å]	2.310	2.310
Rmerge	0.155	0.560
Number of reflections	17710	1763
<I/σ(I)>	10.04	2.14
Completeness [%]	95.8	80.9
Redundancy	6.39	2.88
CC(1/2)	0.989	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298.15	0.2M LiSo4 Monohydrate, 0.1M Sodium acetate pH-5.0, 30% (w/v) PEG 3350