7FHZ

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-02-28
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	47.983, 160.579, 46.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.730 - 2.500
R-factor	0.2563
Rwork	0.255
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.004
RMSD bond angle	0.782
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.190	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.137	0.390
Number of reflections	13694	1338
<I/σ(I)>	10.52	1.87
Completeness [%]	97.4	86.1
Redundancy	6.94	1.69
CC(1/2)	0.971	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298.15	0.2 Ammonium Sulfate, 0.1M Tris-HCl, pH 9.0, 30% (w/v) PEG 4000