7FHX
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-12-26 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.131, 94.737, 160.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.304 - 2.630 |
| Rwork | 0.225 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fhu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.412 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.360 | 2.730 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.160 | 0.518 |
| Number of reflections | 21068 | 1737 |
| <I/σ(I)> | 14.36 | 2.62 |
| Completeness [%] | 91.2 | 71.3 |
| Redundancy | 10.35 | 3.86 |
| CC(1/2) | 0.988 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298.15 | 0.2M LiSo4 Monohydrate, 0.1M sodium acetate trihydrate pH 5.0, 30% (w/v) PEG 3350 |
