7FHW

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-06-07
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.272, 94.668, 160.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.204 - 2.061
Rwork	0.207
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.009
RMSD bond angle	1.530
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	2.160
High resolution limit [Å]	2.060	2.060
Rmerge	0.119	0.434
Number of reflections	45555	5473
<I/σ(I)>	19.58	3.27
Completeness [%]	96.2	89.8
Redundancy	15.34	4.35
CC(1/2)	0.996	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298.15	0.24M LiSo4 monohydrate, 100mM BIS-TRIS, pH5.5, PEG 3350 25%(w/v)