7FHV

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-11-26
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54178
Spacegroup name	P 1
Unit cell lengths	44.513, 58.213, 67.543
Unit cell angles	71.33, 89.94, 74.86

Refinement procedure

Resolution	53.006 - 2.280
Rwork	0.196
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.007
RMSD bond angle	1.429
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.020	2.380
High resolution limit [Å]	2.280	2.280
Rmerge	0.082	0.338
Number of reflections	26004	3005
<I/σ(I)>	9.17	2.16
Completeness [%]	92.3	88.2
Redundancy	2.52	1.05
CC(1/2)	0.992	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298.15	0.2M LiSo4 monohydrate, 0.1M Sodium Cacodylate pH 6.5, 30% (w/v) PEG 8000