7FHU
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-03-26 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.022, 66.804, 103.120 |
| Unit cell angles | 90.00, 94.39, 90.00 |
Refinement procedure
| Resolution | 43.854 - 2.430 |
| Rwork | 0.227 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qaz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.227 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.890 | 2.530 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.123 | 0.442 |
| Number of reflections | 22678 | 2531 |
| <I/σ(I)> | 19.97 | 5.11 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.66 | 11.4 |
| CC(1/2) | 0.998 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298.15 | 0.2M lithium sulfate monohydrate, 0.1M Tris-HCl pH-8.5, 30% (w/v) PEG 4000. |
