7FHF

Structure of prenyltransferase mutant V49W/Y288F/Q295F from Streptomyces sp. (strain CL190)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95373
Spacegroup name	C 1 2 1
Unit cell lengths	89.321, 43.996, 87.988
Unit cell angles	90.00, 111.62, 90.00

Refinement procedure

Resolution	28.010 - 2.500
R-factor	0.1891
Rwork	0.185
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.170	2.290
High resolution limit [Å]	2.220	2.220
Rmerge	0.270	1.694
Rmeas	0.293	1.854
Rpim	0.112	0.740
Total number of observations	105379
Number of reflections	15706	1210
<I/σ(I)>	4.6
Completeness [%]	98.4	83.4
Redundancy	6.7	6
CC(1/2)	0.987	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v PEG 20,000