7FHD
Structure of prenyltransferase mutant Y288P from Streptomyces sp. (strain CL190)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-03 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 1 |
Unit cell lengths | 44.286, 75.162, 87.605 |
Unit cell angles | 89.94, 89.90, 90.00 |
Refinement procedure
Resolution | 29.200 - 3.500 |
R-factor | 0.2466 |
Rwork | 0.243 |
R-free | 0.31300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zb6 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.262 | 0.446 |
Rmeas | 0.339 | 0.578 |
Rpim | 0.211 | 0.362 |
Total number of observations | 29344 | |
Number of reflections | 13654 | 3344 |
<I/σ(I)> | 2.2 | |
Completeness [%] | 95.8 | 97.7 |
Redundancy | 2.1 | 2.2 |
CC(1/2) | 0.914 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v PEG 4,000 |