7FHC

Structure of prenyltransferase mutant V49W from Streptomyces sp. (strain CL190)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	44.339, 78.750, 85.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.800 - 2.060
R-factor	0.1871
Rwork	0.185
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.340	2.110
High resolution limit [Å]	2.060	2.060
Rmerge	0.340	1.842
Rmeas	0.354	1.919
Rpim	0.097	0.528
Total number of observations	252772
Number of reflections	19285	1457
<I/σ(I)>	6.9
Completeness [%]	99.9	98.7
Redundancy	13.1	12.6
CC(1/2)	0.992	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v PEG 20,000