7FH9
chlorovirus PBCV-1 bi-functional dCMP/dCTP deaminase bi-DCD with dTTP/dTMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2797 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 87.239, 87.239, 87.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.873 - 1.900 |
| R-factor | 0.1706 |
| Rwork | 0.168 |
| R-free | 0.19160 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.873 | 1.931 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.115 | 0.711 |
| Rpim | 0.159 | |
| Number of reflections | 29462 | 1235 |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.8 | |
| CC(1/2) | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH 7.2, 50% MPD, 2.5mM MgCl2, 2.5mM dTTP |
