7FH4
Chlorovirus PBCV-1 bi-functional dCMP/dCTP deaminase bi-DCD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2797 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.582, 81.582, 80.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.791 - 1.996 |
| R-factor | 0.1744 |
| Rwork | 0.170 |
| R-free | 0.21370 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.791 | 2.020 |
| High resolution limit [Å] | 1.996 | 1.996 |
| Rmeas | 0.106 | |
| Rpim | 0.033 | |
| Number of reflections | 40426 | 1216 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 9.3 | |
| CC(1/2) | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 0.1M sodium acetate PH 5.8, 4% PEG 4000, 12% MPD, 2.5mM MgCl2, 2.5mM dCTP |
