7FGV
H/D exchanged Hen egg-white lysozyme denatured in heat condition and refolded in solution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2018-02-16 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.150, 79.150, 37.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.180 - 2.000 |
Rwork | 0.224 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6k8g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.835 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.139 |
Number of reflections | 8521 | 749 |
<I/σ(I)> | 42.1 | |
Completeness [%] | 98.7 | |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | NACL, SODIUM ACETATE-D3, ACETIC ACID-D4, 100% D2O, PH 4.6, VAPOR DIFFUSION, SITTING DROP | |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | NACL, SODIUM ACETATE-D3, ACETIC ACID-D4, 100% D2O, PH 4.6, VAPOR DIFFUSION, SITTING DROP |