7FGP
Crystal structure of Aureimonas altamirenisis flavin-containing opine dehydrogenase (FAD-bound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.970000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.727, 105.727, 261.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.018 - 1.470 |
R-factor | 0.1742 |
Rwork | 0.174 |
R-free | 0.18530 |
Structure solution method | SAD |
Data scaling software | Aimless (0.7.4) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.020 | 49.020 | 1.500 |
High resolution limit [Å] | 1.470 | 8.050 | 1.470 |
Rmerge | 0.635 | 0.327 | 2.115 |
Rmeas | 0.640 | 0.329 | 2.139 |
Rpim | 0.076 | 0.040 | 0.321 |
Total number of observations | 10079189 | 71123 | 312964 |
Number of reflections | 146819 | 1103 | 7175 |
<I/σ(I)> | 10.1 | 18.1 | 2.3 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 68.7 | 64.5 | 43.6 |
CC(1/2) | 0.987 | 0.987 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Lithium sulfate, 0.1 M Bis-tris pH 5.5, 24.5% PEG 3350 |