7FGN

The crystal structure of the FAF1 UBL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	CCD
Collection date	2013-01-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00000
Spacegroup name	P 42 21 2
Unit cell lengths	65.028, 65.028, 33.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.825 - 1.199
R-factor	0.2232
Rwork	0.222
R-free	0.24550
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	1.240
High resolution limit [Å]	1.199	2.590	1.200
Rmerge	0.050	0.041	0.216
Number of reflections	23078	2472	2256
<I/σ(I)>	19.9
Completeness [%]	99.7	98.6	99.6
Redundancy	12.2	13.6	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Bis-Tris pH 6.5 50 mM (NH4)2SO4 30 % v/v pentaerythritol ethoxylate