7FGM
The complex crystals structure of the FAF1 UBL1_L-Hsp70 NBD with ADP and phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2013-05-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.335, 103.335, 127.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.763 - 2.200 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s3x |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.098 | 0.047 | 0.322 |
Total number of observations | 656487 | ||
Number of reflections | 40339 | 4236 | 3957 |
<I/σ(I)> | 7 | ||
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 16.3 | 18.9 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200 mM Imidazole-malate pH 8.5 12 % PEG 10,000 |