7FGD

A naturally-occurring neuraminidase-inhibitors-resistant NA from asiatic toad influenza B-like virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-01
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	119.991, 142.275, 120.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.350 - 2.100
R-factor	0.2054
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9s
RMSD bond length	0.008
RMSD bond angle	0.988
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rpim	0.031	0.389
Number of reflections	60226	5980
<I/σ(I)>	11.7	2
Completeness [%]	99.9
Redundancy	13.2
CC(1/2)	0.997	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate pH 5.0, 26% v/v polyethylene 200