7FGA

Alpha-1,2-glucosyltransferase_UDP_sucrose_tll1591

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 65
Unit cell lengths	157.712, 157.712, 187.803
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 3.200
R-factor	0.2939
Rwork	0.292
R-free	0.32720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fg9
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.190	0.990
Number of reflections	43413	6364
<I/σ(I)>	6.3
Completeness [%]	99.4
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	ammonium sulfate