7FG9

Alpha-1,2-glucosyltransferase_UDP_tll1591

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 63
Unit cell lengths	177.643, 177.643, 57.034
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.230 - 2.660
R-factor	0.1819
Rwork	0.178
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oy2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.230	2.790
High resolution limit [Å]	2.660	2.660
Rmerge	0.073	0.505
Number of reflections	28981	3875
<I/σ(I)>	12.8
Completeness [%]	98.1
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	phosphate