7FG6

Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2012-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	52.639, 52.639, 263.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.590 - 2.800
R-factor	0.284
Rwork	0.282
R-free	0.32380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j1u
RMSD bond length	0.006
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.590	2.950
High resolution limit [Å]	2.800	2.800
Rpim	0.072	0.566
Number of reflections	11299	1606
<I/σ(I)>	14
Completeness [%]	100.0	100
Redundancy	10.3	10.6
CC(1/2)	0.997	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297.15	0.2 M Magnesium acetate, 0.1 M Sodium cacodylate pH6.5, 20% (w/v) PEG 8,000