7FFT

The crystal structure of a domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	88.937, 89.406, 64.022
Unit cell angles	90.00, 114.92, 90.00

Refinement procedure

Resolution	36.390 - 1.600
R-factor	0.1886
Rwork	0.188
R-free	0.20650
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.390	1.656
High resolution limit [Å]	1.599	1.599
Number of reflections	58575	2661
<I/σ(I)>	36.4596
Completeness [%]	97.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	279	50% (v/v) 2-Methyl-2, 4-pentanediol (MPD) and 0.1 M HEPES (pH 7.5)