7FF8

Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	200
Detector technology	CCD
Collection date	2019-08-07
Detector	RAYONIX MX300-HS
Wavelength(s)	1.04
Spacegroup name	C 1 2 1
Unit cell lengths	140.264, 49.928, 62.381
Unit cell angles	90.00, 100.14, 90.00

Refinement procedure

Resolution	34.520 - 2.800
R-factor	0.2721
Rwork	0.269
R-free	0.32650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oz6
RMSD bond length	0.010
RMSD bond angle	1.353
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.300	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	21209	2078
<I/σ(I)>	13.46
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.975	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.6M Ammonium Sulphate, 0.1M Bicine