7FF1
Structure of C34E136G/N36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.089, 50.391, 56.909 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 43.740 - 1.689 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aik |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.401 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.740 | 1.790 |
| High resolution limit [Å] | 1.689 | 1.689 |
| Rmerge | 0.043 | 0.976 |
| Number of reflections | 28119 | 4478 |
| <I/σ(I)> | 19.95 | 1.45 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.628 | |
| CC(1/2) | 0.999 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | BIS-Tris,Polyethylene glycol monomethyl ether 5000 |
