7FEX
Crystal structure of Sus scrofa Schlafen11 N-terminal domain (2.69 A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.991, 64.991, 344.018 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.560 - 2.690 |
R-factor | 0.2177 |
Rwork | 0.215 |
R-free | 0.25840 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.564 | 2.710 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.143 | 1.150 |
Number of reflections | 22498 | 3610 |
<I/σ(I)> | 26.33 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 34.4 | 34.8 |
CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES pH7.5, 22%PEG3350, 0.15M Lithium sulfate |