7FEF
Crystal structure of AtMBD6 with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 32 |
| Unit cell lengths | 40.316, 40.316, 83.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.910 - 2.390 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c1a 2yk8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.910 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 6926 | 692 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 10.3 | |
| CC(1/2) | 0.989 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M Bis-tris pH 6.5, 25% PEG 3350 |
